R2a Agar Plates

Tryptone peptone acicase and yeast extract provides necessary carbon nitrogen sources vitamins minerals and growth.

R2a agar plates.

Science lab agar media. R2a agar was developed by reasoner and geldreich for bacterial plate counts of treated potable water. R2a agar reasoner s 2a agar is a culture medium developed to study bacteria which normally inhabit potable water. The soil agar suspensions 200 μl plate were plated directly onto double thick 30 ml per plate 0 5 tsa bd difco becton dickinson and company sparks md usa or r2a agar bd difco plates.

R2a agar as compared to other media recommended for the heterotrophic plate count hpc contains reduced levels of peptone yeast extract and dextrose. As compared to tryptone glucose agar or standard methods agar r2a agar has been reported to give improved recovery of stress and chlorine tolerant bacteria from drinking water systems 2 5 7. 1 product result match criteria. Summary and explanation r2a agar was developed by reasoner and geldreich of the u s.

Product name mxsmcra48. Milliflex cassette prefilled with r2a agar. R2a agar for the cultivation of bacteria from water samples 15x100mm plate order by the package of 10 by hardy diagnostics. R2a agar a nonspecific medium imitates water so is used for water analysis.

It is frequently the base medium of other agar types. R2a agar is a low nutrient medium and in combination with a lower incubation temperature and longer incubation time stimulates the growth of stressed and chlorine tolerant bacteria. R 2a agar is a modification of this medium 2 3 r 2a agar is an alternative medium used for the heterotrophic plate counts and for subculturing isolates from potable waters 1 r 2a agar is also recommended by apha 4 for pour plate spread plate and membrane filter technique r 2a. This low nutrient agar is used for the recovery of stressed heterotrophic plate count bacteria found in various types.

For example blood agar plates are made by enriching tsa plates with blood. R2a agar intended use remel r2a agar is a solid medium recommended for use in qualitative and quantitative procedures for performing heterotrophic plate counts of treated potable water. Since its development in 1985 it has been found to allow the culturing of many other bacteria that will not readily grow on fuller complex. The decreased nutrient level along with the addition of sodium pyruvate enhances the recovery of many stressed and chlorine tolerant bacteria present in treated waters.

Tryptic trypticase soy agar tsa is a general purpose medium produced by enzymatic digestion of soybean meal and casein. These bacteria tend to be slow growing species and would quickly be suppressed by faster growing species on a richer culture medium.